What is Bstfa used for?
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What is Bstfa used for?
N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) is a chemical compound that is used to derivatise labile groups such as hydroxyl on other chemicals, with the more stable trimethylsilyl group, which protects the labile group and allows the compound to be used for analytical purposes or as a chemical reagent for …
What is silylation reaction?
Silylation is the introduction of one or more (usually) substituted silyl groups (R3Si) to a molecule. The process is the basis of organosilicon chemistry.
Why is derivatization used in GC?
The derivatization is typically done to change the analyte properties for a better separation and also for enhancing the method sensitivity. In GC/MS, derivatization may improve the capability of compound identification.
Why is chemical derivatization needed?
In mass spectrometry, derivatization allows one to obtain more information about the structure of the analyzed molecule, like the position of a particular functional group in the molecule.
Why is derivatization necessary?
The derivatization is typically done to change the analyte properties for a better separation and also for enhancing the method sensitivity. In GC/MS, derivatization may improve the capability of compound identification. Examples illustrating such improvements are included.
What does silyl mean?
Definition of silyl : the univalent radical SiH3 derived from monosilane by removal of one hydrogen atom.
Why is derivatization used?
Derivatization is the process of chemically altering an analyte or analytes. Laboratorians often choose to derivatize particular analytes in order to improve their chromatography, thermal stability, or their identification. Derivatization for GC-MS typically involves silylation, alkylation, or acylation reactions.
How do you do derivatization?
The three most widely used methods of derivatization in GC are silylation, acylation, and alkylation. Silylation. In this reaction, active hydrogens are displaced by a silyl group, most often tetramethylsilane (TMS). The general reaction scheme is illustrated for TMS reacting with an alcohol below.
How do you do silylation?
The process involves the replacement of a proton with a trialkylsilyl group, typically trimethylsilyl (-SiMe3). Generally the substrate is deprotonated with a suitable strong base followed by treatment with a silyl chloride (e.g. trimethylsilyl chloride).
How do I get rid of silyl group?
Typical deprotection protocols for the removal of silyl ethers are acidic aqueous THF, or acidic methanol, alkaline aqueous solutions and sources of fluoride ion, most commonly tetra-n-butylammonium fluoride, TBAF, in various solvents.
