What is osteogenic media?

What is osteogenic media?

Osteogenic Differentiation Medium is designed to efficiently induce adipose-derived stem cells differentiation into osteoblasts. This medium is fully supplemented and containing 10% fetal bovine serum. This medium is sterile filtered and is ready for use.

What is Stem cell?

Stem cells are the body’s raw materials — cells from which all other cells with specialized functions are generated. Under the right conditions in the body or a laboratory, stem cells divide to form more cells called daughter cells.

How do you make an osteogenic medium?

Finally, the experimental osteogenic medium was prepared by adding 10% FBS, 0.01 μM dexamethasone, 50 μg/mL ascorbic acid, 10 mM sodium β-glycerophosphate, 10,000 U/mL penicillin, and 10,000 U/mL streptomycin to the experimental culture medium.

What makes stem cells unique?

Stem cells are different from other cells in the body in three ways: They can divide and renew themselves over a long time. They are unspecialized, so they cannot do specific functions in the body. They have the potential to become specialized cells, such as muscle cells, blood cells, and brain cells.

What is osteogenic induction?

To efficiently drive differentiation of mesenchymal stem cells (MSCs) to osteocytes.

What is osteogenic differentiation of mesenchymal stem cells?

Mesenchymal stem cell (MSC) osteogenic differentiation was the first differentiation to be identified by Friedenstein et al. in the late 1960s–early 1970s [1, 2]. As the MSCs (known then as marrow stromal cells) were extracted from the bone marrow, it was not surprising that they could give rise to osteoblasts.

What is osteoconductive and osteoinductive?

Osteoinduction implies the recruitment of immature cells and the stimulation of these cells to develop into preosteoblasts. In a bone healing situation such as a fracture, the majority of bone healing is dependent on osteoinduction. Osteoconduction means that bone grows on a surface.

How does osteogenic loading work?

The amount of weight-bearing that causes this response from the bone is called “osteogenic loading” because it takes a certain “load” to stimulate the bone-building cells. In contrast, “unloading” the bones from prolonged bed rest or space travel can result in loss of bone density.

How do you improve osteogenic differentiation?

Mechanical vibrations, stress, and shear forces can all enhance osteogenic differentiation of MSCs while the lack of mechanical stimuli can induce adipogenesis [2,4]. Luu et al. have shown that low magnitude mechanical stimulation (LMMS) induces osteogenesis and inhibit adipogenesis [5,6].

Which stem cells are best?

1. Totipotent (or Omnipotent) Stem Cells. These stem cells are the most powerful that exist. They can differentiate into embryonic, as well as extra-embryonic tissues, such as chorion, yolk sac, amnion, and the allantois.