Is brain heart infusion broth a selective medium?
Table of Contents
Is brain heart infusion broth a selective medium?
Brain Heart Infusion Broth (BHI BROTH) is an enriched non-selective media intended for the cultivation of most anaerobic bacteria and other fastidious microorganisms.
What kind of media is brain heart infusion?
Brain heart infusion (BHI) is a growth medium for growing microorganisms. It is a nutrient-rich medium, and can therefore be used to culture a variety of fastidious organisms. In particular, it has been used to culture streptococci, pneumococci and meningococci, which can be otherwise challenging to grow.
Is brain heart infusion broth differential?
In the formulation containing 6.5% sodium chloride, the salt acts as a differential and/or selective agent by interfering with membrane permeability and osmotic and electrokinetic equilibria in salt-intolerant organisms.
Is brain heart infusion a complex medium?
In complex media, which contain extracts and digests of yeasts, meat, or plants, the precise chemical composition of the medium is not known. Amounts of individual components are undetermined and variable. Nutrient broth, tryptic soy broth, and brain heart infusion, are all examples of complex media.
What is the composition of brain heart infusion BHI agar?
Brain Heart Infusion (BHI) Agar derives its nutrients from the brain heart infusion, peptone and glucose components. Protease peptone and infusions used in the media serve as sources of carbon, nitrogen, vitamins, amino acids, along with essential growth factors. Dextrose is the energy source.
Is BHI selective or differential?
BHI Agar is an enriched non-selective media used for the isolation and cultivation of a wide variety of bacteria, including yeast and molds. The basic nutritive properties of this media are brain heart infusion from solids, meat peptone, and yeast extract.
Why is brain and heart infusion agar used?
Brain Heart Infusion Agar is a general purpose plating medium used for the isolation, cultivation, and maintenance of a variety of fastidious and non- fastidious microorganisms.
What are examples of selective media?
Examples of selective media:
- Eosin methylene blue contains dyes that are toxic for Gram-positive bacteria.
- YM (yeast extract agar) has a low pH, deterring bacterial growth.
- MEA (malt extract agar) has a low pH, deterring bacterial growth.
- MacConkey agar is for Gram-negative bacteria.
What is an example of a differential media?
Examples of differential media include: Blood agar (used in strep tests), which contains bovine heart blood that becomes transparent in the presence of hemolytic. Streptococcuseosin methylene blue (EMB), which is differential for lactose and sucrose fermentation.
Which media is differential?
Differential media contain compounds that allow groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media, usually on the basis of some biochemical difference between the two groups.
Which media are selective and differential?
MacConkey agar is an example of a medium that is both differential and selective. The presence of bile salts, as well as crystal violet, within the media prevent gram-positive organisms from growing.
Which media is selective and differential?
Is blood agar selective or differential or both?
differential medium
Blood agar is a differential medium that distinguishes bacterial species by their ability to break down red blood cells. The ability to break down the cells will cause a change in the color of the blood agar. Some media are both differential and selective.
How do you know if a medium is selective or differential?
Key Points
- Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms.
- Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present.
