How do you use FuGENE?
Table of Contents
How do you use FuGENE?
Briefly vortex or mix the FuGENE® HD Transfection Reagent/DNA complex. Add 2–10µl of complex per well to 96-well plate containing 100µl of cells in growth medium. Mix by pipetting or using a plate shaker for 10–30 seconds. Return plates to the incubator.
What is in FuGENE 6 transfection reagent?
FuGENE® 6 Transfection Reagent provides sturdy results in a wide variety of standard laboratory cell lines, making it ideal for everyday use. The simple protocol eliminates tedious culture medium changes and eliminates a source of variation.
What concentration should I use for siRNA?
At what concentration should I make my siRNA stock solution? We recommend a concentration of 50 – 100 µM.
How do you mix siRNA?
To dilute the 5x siRNA Buffer to 1x siRNA Buffer, mix four volumes of sterile RNase-free water with one volume of 5x siRNA Buffer. The composition of the 1x siRNA Buffer is 60 mM KCl, 6 mM HEPES-pH 7.5, and 0.2 mM MgCl2.
How does FuGENE HD work?
FuGENE® HD interacts with nucleic acids to provide efficient and safe entry into the cell, allowing users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells. Its simple, easy-to-use protocol allows users to free up valuable lab time.
Is FuGENE lipid based?
The FuGENE HD Transfection Reagent is lipid-based, simple-to-use, and can result in high transfection efficiencies with minimal cyototoxicity.
What is FuGENE HD?
The FuGENE® HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity.
How do you dilute siRNA?
What is FuGENE transfection reagent?
How does FuGENE transfection reagent work?
FuGENE® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA, then transports the complex into animal or insect cells. It is suitable for use in media with or without serum, and for transient or stable transfection, as well as co-transfections of multiple DNA plasmids.
What is the difference between stable and transient transfection?
In stable transfection, the plasmid DNA successfully integrates into the cellular genome and will be passed on to future generations of the cell. However, in transient transfection, the transfected material enters the cell but does not get integrated into the cellular genome.
What can you resuspend siRNA in?
Resuspended siRNA should be stored at -20 °C in a manual defrost or non-cycling freezer. Storage at 4 °C is suitable for up to 6 weeks. Synthetic RNAi reagents should be resuspended in RNase-free solutions. We recommend 1x siRNA Buffer (diluted from 5x siRNA Buffer, Cat #B-002000-UB-100).
