What is Bio-Plex 200?
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What is Bio-Plex 200?
The Bio-Plex 200 is an automated immunoassay multiplex array system that provides a wide range of biomarker assessments, including cytokines, chemokines, growth factors, soluble receptors, hormones, and gene expression analyses.
How does BioPlex work?
The technology makes use of multiple assays whereby one antibody to a specific analyte is attached to a set of beads with the same colour and the second antibody is used to quantify the bound antigen. The use of different coloured beads enables the simultaneous detection of many other analytes in the same sample.
What is a BioPlex?
BioPlex (biophysical interactions of ORFeome-based complexes) is an open access resource for studying protein-protein interactions. It is the result of collaborations between Harvard Medical School and Biogen. BioPlex 1.0 reported 23,744 interactions among 7,668 proteins.
What is a Bioplex assay?
Bio-Plex Multiplex Immunoassays allow you to simultaneously quantify up to 500 proteins, peptides, and nucleic acid targets in a single 12.5 µl sample. Assays for over 450 biologically relevant targets are available as ready-to-use premixed multiplex panels and singleplex sets or in custom-mixed panels.
What is xMAP technology?
Luminex xMap technology is a bead-based multiplexed immunoassay system in a microplate format. The system can simultaneously detect many targets in a single sample — up to 500, depending on system design. The microspheres used in the Luminex system have different spectral addresses (color codes).
What is multiplex immunoassay?
Multiplex assays are a type of immunoassay capable of simultaneous measurement of multiple analytes. Multiplex assays are derived from the ELISA format, but instead of producing a single signal measurement, they produce multiple signal measurements.
How does Luminex 200 work?
Phycoerythrin (PE)-conjugated streptavidin is added. It binds to the biotinylated detection antibodies. Beads are read on a dual-laser flow-based detection instrument, such as the Luminex 200™ or FlexMap® analyzer. One laser classifies the bead and determines the analyte that is being detected.
What is multiplex test?
For certain patients, such as those presenting with multiple symptoms associated with respiratory viral infections, multiplex testing allows providers to test for multiple viruses at once and provide a more comprehensive clinical diagnosis.
What is multiplex biology?
Multiplex PCR is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment. In a multiplexing assay, more than one target sequence can be amplified by using multiple primer pairs in a reaction mixture.
How do you analyze data Luminex?
To analyze and perform quality control on Luminex data, a lab might follow these steps:
- Set up an assay project.
- Create an assay design.
- Import data and enter metadata.
- Explore results.
- Perform within-run quality control.
- Perform cross-run quality control.
- Share quality-controlled data with collaborators.
How does the Luminex assay work?
Using a Luminex instrument, beads are excited by one laser to determine the bead region and corresponding assigned analyte. Another laser determines the magnitude of the PE-derived signal, which is proportional to the amount of analyte bound. Multiple readings are taken at each bead region, ensuring robust detection.
What does high Plex mean?
Generally, smaller targets are more efficiently amplified than larger ones (18, 19). Hi-Plex defines amplicon sizes within a narrow size range, thus eliminating size-related bias.
What is multiplex PCR analysis?
Multiplex PCR is the simultaneous detection of multiple targets in a single reaction well, with a different pair of primers for each target. This technique requires two or more probes that can be distinguished from each other and detected simultaneously.
What is a Luminex assay?
A Luminex assay is a type of immunoassay that precisely measures multiple analytes in one sample. The Luminex® xMAP® technology is a bead-based immunoassay that allows for multiplex detection of up to 100 analytes simultaneously.
What is Luminex used for?
Luminex Platform Luminex multiplex assays use xMAP bead-based technology to simultaneously detect and quantitate multiple secreted proteins including cytokines, chemokines, and growth factors. This multiplex technology can also be used to measure gene expression utilizing the Invitrogen QuantiGene Plex assays.
What does a Luminex 200 do?
The Luminex 200 Instrument System sets the standard for multiplexing, providing the ability to perform up to 100 different tests in a single reaction volume on a flow cytometry-based platform.
What is the difference between a Singleplex and a multiplex PCR?
In conventional singleplex PCR, a single target is amplified in a single reaction tube. In contrast, multiplex PCR allows for simultaneous amplification of multiple target sequences in a single tube using specific primer sets in combination with probes labeled with spectrally distinct fluorophores.
