What is an example of a colorimetric assay?

What is an example of a colorimetric assay?

Colorimetric assays They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.

What is meant by colorimetric assay?

Colorimetric analysis refers to a quantitative technique used to measure the concentration of a given substance in a solution. This allows the quantification of substances such as water and chemicals on metallic surfaces and their corresponding contribution to corrosion rates.

What Colour are paracetamol tablets?

The capsules are blue and white in colour with “S174” printed in white ink on the capsule. It contains white powder. Paracetamol 500mg Capsules come in cartons of 48, 60, 96 and 100 capsules.

Why is colorimetric assay important?

Colorimetric Assays This allows precise determination of the enzyme activity by optical density. For example, peroxidase activity is measured by the reduction of the substrate in the presence of hydrogen peroxide, producing a soluble chromogen that is measured with a spectrophotometer.

How do you do colorimetric analysis?

Step 1: Determine the wavelength (colour) of light to use for the colorimetric analysis. Step 2: Prepare a set of standard solutions of known concentration. Step 3: Measure the absorbance of each standard solution using the colorimeter. Step 4: Plot the absorbance vs concentration for each standard solution on a graph.

How do you do a colorimetric test?

Insert the tube into the colorimeter and follow the instructions for performing a reagent blank in your meter manual. Remove the tube, dispose of the reagent blank, and use the same tube to run the test. Running a reagent blank eliminates errors that may result from small differences in reagent color.

What is the composition of paracetamol tablet?

The active substance is paracetamol. Each tablet contains 500mg of paracetamol. The other ingredients are, maize starch, potassium sorbate, purified talc, stearic acid, povidone, and soluble starch.

What is the excipient in paracetamol?

Hypromellose is one of the main excipients commonly used for preparation of solid formulations with paracetamol.

What is the principle of colorimetric analysis?

Principle of Colorimeter It is a photometric technique which states that when a beam of incident light of intensity Io passes through a solution, the following occur: A part of it is reflected which is denoted as I. A part of it is absorbed which is denoted as I. Rest of the light is transmitted and is denoted as I.

What are the advantages of colorimetric analysis?

Compared with other methods, the colorimetric method has some obvious advantages, such as low cost, simple instruments (or, in the case of naked eye detection, no instruments), and can be qualitatively or semiqualitatively identified by the naked eye. However, colorimetry is generally less sensitive.

How does colorimetric method work?

In a colorimeter, a beam of light with a specific wavelength is passed through a solution via a series of lenses, which navigate the colored light to the measuring device. This analyzes the color compared to an existing standard. A microprocessor then calculates the absorbance or percent transmittance.

Why are colorimetric assays important?

Numerous colorimetric assays exist for the measurement of analytes, ranging from simple pH measurements (1) to more complex assays for pesticides (2−5) and pharmaceutical compounds, (6,7) which can be valuable in applications such as water testing and health monitoring.

What is the purpose of colorimetric analysis?

Colorimetric analysis is the technique normally used to determine the concentration of analyte through comparing the color changes of the solution.

How do you synthesis paracetamol?

Synthesis of Paracetamol (Morning) Paracetamol is made by reacting 4-aminophenol with ethanoic anhydride (more commonly called acetic anhydride). This reaction forms an amide bond and ethanoic acid as a by- product. When the reaction is complete the paracetamol is then isolated and purified.

Why is sorbitol added to paracetamol?

With sorbitol in paracetamol tablets, it has been suggested that the improved absorption is due to the sorbitol acting on the metabolism of paracetamol and as a dispersing agent (Gwilt, Robertson & others, 1963).

Why are paracetamol tablets prepared wet granulation?

Granulation strategy will improve stream and weight characteristics, reduce confinement, improve content consistency, and discard unrestrained proportions of fine particles. Herbal drugs can also be incorporated into granules so as to prepare herbal drug tablets.

What is the purpose of colorimeter?

A colorimeter is an instrument that compares the amount of light getting through a solution with the amount that can get through a sample of pure solvent. A colorimeter contains a photocell which is able to detect the amount of light passing through the solution under investigation.