How many types of capillary electrophoresis are there?
Table of Contents
How many types of capillary electrophoresis are there?
There are six types of capillary electroseparation available: capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), micellar electrokinetic capillary chromatography (MEKC), capillary electrochromatography (CEC), capillary isoelectric focusing (CIEF), and capillary isotachophoresis (CITP).
What is the difference between capillary electrophoresis and gel electrophoresis?
Capillary Electrophoresis vs Gel Electrophoresis Capillary electrophoresis is a technique which separates biomolecules on a capillary tube using a liquid or gel polymer medium. Gel electrophoresis is a technique which separates biomolecules on a vertical or horizontal plane using a polymer gel medium.
Which type of capillary electrophoresis is most commonly used?
Capillary isoelectric focusing (CIEF) This form of capillary electrophoresis is commonly employed to separate peptides and proteins.
What is the process of capillary electrophoresis?
Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution.
What is capillary zone electrophoresis?
Capillary zone electrophoresis (CZE) is a technique successfully used for the separation of proteins, peptides, and nucleic acids. Other applications where CZE may be useful include analysis of inorganic anions and cations, such as those typically separated by ion chromatography.
How does pH affect capillary electrophoresis?
The lower pH of the sieving buffer may induce the polymer more efficiently to adsorb on the capillary wall to suppress EOF and DNA-capillary wall interaction for DNA separation.
Why is buffer used in capillary electrophoresis?
Biological buffers are an essential part of electrophoresis techniques. They are usually added to solutions in order to make the pH stable while a current is carried through the sample. Additionally, buffers provide the ions necessary for electrophoretic migration.
What buffers are used in capillary electrophoresis?
With this technique two kinds of buffers are used. One buffer with high mobility as a leading buffer and one buffer with very low mobility as a terminating buffer. The mobility of the sample components must be between the leading and terminating.
What is zeta potential in capillary electrophoresis?
The zeta potential, ΞΎ, also known as the electrokinetic potential is the electric potential at the interface of the double layer. Hence, in our case, it is the potential of the diffuse layer that is at a finite distance from the capillary wall.
