What is Myc for?
Table of Contents
What is Myc for?
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism.
How does Myc tag work?
A Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be fused to either the C- or N-terminus of a protein through recombinant DNA technology. It can facilitate the affinity purification of the tagged protein, as well as the isolation and detection of the tagged protein.
How many kDa is Myc?
The c-Myc gene encodes a polypeptide with a predicted molecular weight of 49 kDa but also demonstrates aberrant electrophoretic mobility in western blotting to manifest an apparent molecular weight of around 62 kDa (p62c-myc) [7].
Is Myc an antibody?
c-Myc Monoclonal Antibody (9E10)
What is anti c-myc?
Anti-c-myc is a monoclonal antibody to c-myc peptide (clone 9E10). Anti-c-myc recognizes the 9E10 epitope sequence (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins.
How many amino acids is a Myc tag?
10 amino acids
The Myc-tag contains 10 amino acids (aa) and the sequence is EQKLISEEDL.
What is peptide tag?
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.
How much does Myc tag weigh?
c-Myc tag peptide is a synthetic peptide with a molecular weight of 1,203 Da. The peptide sequence is EQKLISEEDL, corresponding to the C-terminal amino acids (410-419) of human c-myc protein.
What type of protein is myc?
1. C-Myc. C-Myc protein is a member of a family of proteins that regulate cell proliferation and apoptosis. The ability of c-myc to regulate apoptotic cell death results from the coordinated activation of c-Myc and several protein partners (such as Max) that facilitate DNA binding and activate transcription.
