What is promoter fusion?

What is promoter fusion?

Abstract. One important tool in molecular biology takes advantage of promoter fusions where the expression of one gene is controlled by the promoter or regulatory elements of another gene. Promoter fusions engineered in vitro can help to determine how gene expression is regulated in cells and whole organisms.

What is an example of fusion gene?

For example, the BCR-ABL fusion gene and protein are found in some types of leukemia. Fusion genes and proteins may also be found in several other types of cancer, including soft tissue sarcoma, cancers of the prostate, breast, lung, bladder, colon, and rectum, and CNS tumors.

How are reporter genes inserted?

To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. For bacteria or prokaryotic cells in culture, this is usually in the form of a circular DNA molecule called a plasmid.

What is a GFP fusion protein?

The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

How does gene fusion work?

Gene fusions are hybrid genes formed when two previously independent genes become juxtaposed. The fusion can result from structural rearrangements like translocations and deletions, transcription read-through of neighboring genes (1–3), or the trans- and cis-splicing of pre-mRNAs (4–8) (Figure ​ 1).

What is reporter gene fusion?

A reporter fusion is the hybrid of a gene or portion of a gene with a tractable marker.

What is GFP fusion used for?

INTRODUCTIONGFP (green fluorescent protein) fusion proteins have been used to address a wide range of questions in individual cells, as well as in tissues of a particular organism. GFP fusion proteins can be transiently or stably expressed.

How do you Visualise GFP?

We find that GFP fluorescence survives fixation in 4% paraformaldehyde/0.1% glutaraldehyde and can be visualized directly by fluorescence microscopy in unstained, 1 microm sections of LR White-embedded material.

Where are fusion proteins made?

Naturally occurring fusion proteins are commonly found in cancer cells, where they may function as oncoproteins.

What is fusion mutation?

Gene fusions, or translocations, resulting from chromosomal rearrangements are the most common mutation class. They lead to chimeric transcripts or to deregulation of genes through juxtapositioning of novel promoter or enhancer regions.

How do you fuse two genes?

The quick way to fuse you two gene with a linker of (GGGGS)3 by digestion is, 1) do a PCR amplification of your 2nd gene with a long forward primer containing: the restriction enzyme site (the same used for cloning the 3’end of your first gene), your (GGGGS)3 coding sequence and the sequence matched to the 5’end of …

Is a gene fusion a mutation?